Infection of the urinary tract is one. of the most common bacterial disease. Many resistant strains, causing urinary tract infection have increased gradually, the urinary tract infection is difficult to cure. In urinary tract infection, bacterial culture and antibiotic sensitivity test are important in clinical management. Widely used disc method is a convenient routine method and is proven as proper method for selection of antibiotics, but it is no guidance for proper dosage of antibiotics.
This study. deals with epidemiological review of the patient and the bacteriological examination on 208 strains, isolated from urine specimen that were referred to the department of clinical pathology for bacterial culture from June to August, 1981. Among these pathogens, the most prevalent one was E.. coli. And 110 strains of E. coli were examined as disc method and agar dilution method for antibiotics susceptibility test.
The results are as follows:
1. By far the most common pathogens were the E. coli (59. 1 %) and lesser extend, K pneumoniae (17.8 %), Enterococci (11 %) , E. cloacae (6. 3 %) and Pseudomonas sp(5.8%).
2. The sexual incidence of male to female revealed as 1 : 3.
3. Important disorders causing urinary tract infection were indwelling catheter(complicated in urethritis and cystitis) of 42 cases (20.2 %), Acute pyelonephritis of 31 cases (3.4%), cystitis of 7 cases (3.4%), and nephrotic syndrome, of 6..-cases (2.9%) etc.
4. The result of agar dilution method of 110. strains of E.´ coli disclosed 96.4 % were inhibited by 4 mcg/ml amikacin, 92. 7% by 1 mcg/ml gentamicin, 90. 9/o by 2 mcg/ml tobramycin, 93. 6 % by 64 mcg/ml cephalothin and 16. 4 % by 32 mcg/ml carbenicillin. In spite of those antibiotics such as amikacin, gentamycin revealed high percentage of susceptibility in low concentrations, cephalothin and carbenicillin showed high percentage of resistance even in high concentrations.
5. The disc method. and agar dilution method showed similar results in comparison of inhibition rate of bacterial. growth in each antibiotics.
6. RUA according to the time and temperature changes, (the urine standing at room temperature) sugar was disappeared after 24 hrs. especially in case of many bacteria were shown in microscopic examination.
7. Colony count according to the time, temperature and media changes, no colony was observed at 4°C and showed increasing colony count as time lasting at 28°C or 37°C. In latter case, no difference was noted between urine and BHI broth. After 24 hrs, no colony was observed at 24°C and innumerable were at 28°C and 37°C. It should be emphasized that the culture of causative agents of urinary tract infection are susceptiptible to influences from the method of specimen collection and storage or transfer condition before the testing.
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